Home > Sandwich ELISA Kits > Human Monocyte To Macrophage Differentiation Associated Protein (MMA) ELISA Kit

Product Name:

Human Monocyte To Macrophage Differentiation Associated Protein (MMA) ELISA Kit

Cat.#:

JL52510

Regulatory Status:

RUO

Alternative:

MMD; PAQR11; Progestin and adipoQ receptor family member 11

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.05ng/mL

Specificity:

This kit recognizes Human MMA in samples. No significant cross-reactivity or interference between Human MMA and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MMA

Monocyte to macrophage differentiation factor also known as PAQR11 is a protein that in humans is encoded by the MMD gene. It is a member of the progestin and adipoQ receptor family. PAQR11 is a scaffold protein that is localized to the Golgi apparatus.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.36	1.56	1.04	0.65	0.46	0.34	0.29	0.1
Corrected OD	2.26	1.46	0.94	0.55	0.36	0.24	0.19	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MMA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	102
Cell Lysates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human MMA and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	108